Synthesis and properties of oligonucleotides containing 2'-O-methyl-2-thiouridine. |
| |
| Authors: | K Shohda I Okamoto T Wada M Sekine |
| |
| Affiliation: | Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan. |
| |
| Abstract: | New methods to synthesize 2'-O-methyl-2-thiouridine and its phosphoramidite building block for incorporation into oligonucleotides were developed. Oligonucleotides containing 2'-O-methyl-2-thiouridine were expected to be favorable as antisense agents in several respects, i.e., nuclease resistance, stable RNA duplex formation, and exact base recognition. Therefore, to make them clear, we synthesized oligonucleotides having 2'-O-methyl-2-thiouridine and analyzed their properties in detail. |
| |
| Keywords: | |
|
|
