Induction and identification of tetraploids using in vitro colchicine treatment of <Emphasis Type="Italic">Gerbera jamesonii</Emphasis> Bolus cv. Sciella |
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Authors: | Saikat Gantait Nirmal Mandal Somnath Bhattacharyya Prakash Kanti Das |
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Institution: | (1) Department of Biotechnology, Instrumentation and Environmental Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, WB, 741252, India;(2) Department of Genetics, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, WB, 741252, India |
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Abstract: | To induce variation through chromosome doubling in Gerbera jamesonii Bolus cv. Sciella, two-week-old in vitro grown shoots were treated with various concentrations of colchicine (0.01, 0.05,
0.10, 0.50 or 1% w/v) for 2, 4 or 8 h. Treated shoots were then cultured on Murashige and Skoog (MS) medium supplemented with
8.8 μM 6-benzyladenine (BA) and 155 μM adenine sulphate (ADS), and subsequently transferred to fresh MS medium containing
2.85 μM indole-3 acetic acid (IAA) for rooting. When shoots were treated with 0.1% colchicine for 8 h, 64% of recovered plantlets
were tetraploid. Ploidy of plantlets was confirmed by flow cytometry, stomatal analysis, and morphological characters. Tetraploid
plantlets displayed slower proliferation along with higher vigor and thickened broad leaves. Moreover, tetraploid plants developed
larger flowers, longer stalks, and have improved vase-life, all contributing to higher ornamental value of gerbera. |
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