The use of duplex-specific crab nuclease for rapid analysis of single-nucleotide polymorphisms and the detection of DNA targets in complex PCR products |
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| Authors: | I A Shagina E A Bogdanova I M Altshuler S A Luk’yanov D A Shagin |
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| Institution: | 1.ZAO Evrogen,Moscow,Russia;2.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences,Moscow,Russia |
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| Abstract: | A rapid method for single-nucleotide polymorphism analysis and the detection of target clones after cloning of complex PCR
products based on the use of duplex-specific crab nuclease and a universal fluorescent probe has been developed. The method
is an alternative to the labor-intensive procedures of clone screening employing radioactively labeled probes, gel-based restriction
analysis, and costly sequencing. The efficiency of the novel method has been demonstrated in a range of model systems. |
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