IN VIVO INHIBITION OF RAT BRAIN PROTEIN SYNTHESIS BY d-AMPHETAMINE |
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Authors: | L. E. Roel M. A. Moskowitz D. Rubin D. Markovitz L. D. Lytle H. N. Munro R. J. Wurtman |
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Affiliation: | Laboratory of Neuroendocrine Regulation and Laboratory of Physiological Chemistry, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. and Section of Neurology, Department of Medicine, Peter Bent Brigham Hospital, Boston, MA 02115, U.S.A. |
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Abstract: | Abstract— Between 1 and 4 h after rats received a single injection of d-amphetamine (15 mg/kg)(when brain polysomes are known to be disaggregated), the in vivo incorporation of [14C]lysine into trichloroacetic acid-precipitable brain protein was reduced by 28–48%. Incorporation of the 14C label into the protein present in a 100,000 g supernatant extract of whole brain was similarly reduced (by 44%). Amphetamine administration suppressed protein synthesis in rat cerebral cortex, cerebellum, hypothalamus, striatum, and brainstem to an equivalent extent. The drug did not significantly affect lysine pool sizes measured in these brain regions; thus the reduced incorporation of labeled lysine was not the result of an isotope dilution effect. We therefore conclude that the brain polysome disaggregation resulting from amphetamine administration is associated with decreased in vivo synthesis of some brain proteins. |
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