Different Effects of Steroidal Therapy on Neuropeptide-Active Enzymes in Male and Female Human Saliva

The hydrolysis of a model neuropeptide (leucine enkephalin) was studied in the presence of saliva obtained from normal and allergic male and female volunteers in the absence and in the presence of steroidal treatment. Possible variations in the formation of substrate hydrolysis by-products were studied in whole samples and after steric exclusion chromatography fractionation. The results obtained confirm already-described variations in substrate hydrolysis in allergic as compared to control saliva, as well as the effect of steroidal treatment on the activity of the substrate-active enzymes. In addition, whereas in male saliva, therapy was associated with a net decrease of substrate hydrolysis, in female saliva hydrolysis remained near the levels measured in the absence of treatment. Finally, therapy induced modifications of enzyme apparent molecular weight distribution that appear to be similar for all substrate-active enzyme classes, but different in male and female saliva. In male saliva, therapy decreased the activity of the enzymes eluted at high apparent molecular weight, while it increased the activity of the enzymes of low apparent molecular weight. Because the increase was considerably less than the decrease, the net effect was to decrease the activity of the substrate-active enzymes, nearly to the low levels measured in the controls. In female saliva the therapy-associated decrease in the activity of the enzymes eluted at high apparent molecular weight was offset by the increase in the activity of those eluted at low apparent molecular weight, consequently, substrate hydrolysis remained near the level measured in the absence of treatment, a level that was higher than that measured in the controls.