Striatal Dopaminergic Fiber Recovery After Acute <Emphasis Type="SmallCaps">l</Emphasis>-DOPA Treatment in 6-Hydroxydopamine (6-OHDA) Lesioned Rats |
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Authors: | Chuanqin Fang Jinbo Yin Zhiqiang Xu Yanjiang Wang Haiwei Xu Huadong Zhou Changyue Gao |
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Institution: | (1) Department of Neurology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China;(2) Department of Basic Medicine, Institute of Physiology, Third Military Medical University, Chongqing, 400038, China;(3) Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China; |
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Abstract: | In order to examine the acute effects of l-DOPA treatment following 6-hydroxydopamine (6-OHDA) injection into rat medial forebrain bundle (MFB). Sprague–Dawley rats
(n = 48) received either 6-OHDA, via intracranial unilateral injection, into the MFB (experimental group) or saline 0.9% (control
group). Administration of l-DOPA or saline 0.9% began 1 month after the 6-OHDA injection for 10 consecutive days. Within 3 days, an increase in the density
of striatal tyrosine hydroxylase (TH) immunoreactive fibers within the striatum, when compared to the control group was observed.
There was no difference in the loss of substantia nigra pars compacta (SNpc) dopaminergic (DA) neurons between. The greater
density of TH fibers in the striatum following l-DOPA may be related to recovery of the DA phenotype and/or sprouting of TH axon terminals. Only animals with severe cell
loss in the SNpc experienced abnormal involuntary movements (AIMs) or “dyskinesias” in response to l-DOPA, which did not correlate with striatal TH fiber density, suggesting that induction of TH-positive fibers does not contribute
to the occurrence of dyskinesia. The relationship between cell loss, fiber density and AIM to the abundance of markers of
microglial activation were also examined. Iba-1, a microglial marker, immunoreactivity was not affected by l-DOPA treatment, was not correlated with the severity of AIM indicating that microglial activation does not contribute to
dyskinetic phenomena. |
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