体内体外培养下飞蝗雄性生殖细胞的分化

在单一TCl99或GRACE培养液中培养的四龄三天东亚飞蝗(Locusta mtgratoria mani lensis精小管，其精子发生只发育至初级精母细胞期，培养液中添加10%小牛血清或飞蝗精巢匀浆液可促使其发育至次级精母细胞期，添加10%分别取自东亚飞蝗蝗蝻、柞蚕蛹及蓖麻蚕蛹的血淋巴可促进其产生约20%的精子。 蜕皮激素及保幼激素对精子的产生无显著影响。移植培养的精小管在受体飞蝗体内不能发育产生精子，注射20μg/虫蜕皮激素可促使其产生大量精子。完整精巢无需注射蜕皮激素即可在受体飞蝗体内发育产生精子。结果表明，昆虫血淋巴内可能含有促细胞分化类因子，此(类)因子可能无种属特异性，外源蜕皮激素可能对精子发生无直接作用，但精子发生同时需要蜕皮激素和血淋巴因子，精巢本身可能有自己的蜕皮激素来源。

IN VITRO AND IN VIVO DIFFERENTIATION OF MALE GERMCELLS IN THE LOCUST LOCUSTA MICRATORIAMSNILENSIS

t was observed that testicular follicles from day 3 fourth instar nymph of theOriental migratory locust Locusta migratoria manilensis cultured in TC199 or Grace's media were arrested at the stage of spermatocyte I. Addition of 10% FBS orlocust testis homogenate could promote development to the stage of spermatocyte II.When cultured in media with 10% hemolymph from either the nymph of L. migratoria manilcnsis or the pupa of Antherea pernyi or Philosamia cynthia ricini, thefollicles developed with massive production of spermatids and 20% sperms. Application of 20--hydroxyecdysone (20-E) and JH--I bad no effect on the development inthe latter case. Testicular follicles of day 3 third instar nymph, day 3 fourth instarnymph and day 0 fifth instar nymph implanted into male or female adult locustsceased development, which was promptly recovered by injection of 20--E. By implantation, intact testis could proceed spermatogenesis and produce sperms spontaneouslywithin & days without injection of ecdysone. These results indicate that the insecthemolymph contains factor(s) that can promote the differentiation of spermatids andspermatogenesis, and the factor(s) seems to be non-species specific. The effect ofecdysteroid might not be direct, but spermatogenesis and normal differentiation ofspermatids need both ecdysteroid and hemolymph factor(s), and the intact testismight have its own source of ecdysone.