Reassessing the role of phospholipase D in the Arabidopsis wounding response |
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Authors: | BASTIAAN O. R. BARGMANN ,ANA M. LAXALT ,BAS TER RIET ,CHRISTA TESTERINK,EMMANUELLE MERQUIOL,ALINA MOSBLECH,ANTONIO LEON-REYES,CORNÉ M. J. PIETERSE,MICHEL A. HARING,INGO HEILMANN,DOROTHEA BARTELS,& TEUN MUNNIK |
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Affiliation: | Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, NL-1098SM, Amsterdam, the Netherlands,;Department of Ecology and Physiology of Plants, Vrije Universiteit Amsterdam, Boelelaan 1085, NL-1081HV, Amsterdam, the Netherlands,;Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany,;Plant-Microbe Interactions, Utrecht University, Padualaan 8, NL-3584CH, Utrecht, the Netherlands and;Universität Bonn, Molekulare Physiologie und Biotechnologie der Pflanzen, Kirschallee 1, D-53115 Bonn, Germany |
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Abstract: | Plants respond to wounding by means of a multitude of reactions, with the purpose of stifling herbivore assault. Phospholipase D (PLD) has previously been implicated in the wounding response. Arabidopsis ( Arabidopsis thaliana ) AtPLD α 1 has been proposed to be activated in intact cells, and the phosphatidic acid (PA) it produces to serve as a precursor for jasmonic acid (JA) synthesis and to be required for wounding-induced gene expression. Independently, PLD activity has been reported to have a bearing on wounding-induced MAPK activation. However, which PLD isoforms are activated, where this activity takes place (in the wounded or non-wounded cells) and what exactly the consequences are is a question that has not been comprehensively addressed. Here, we show that PLD activity during the wounding response is restricted to the ruptured cells using 32Pi-labelled phospholipid analyses of Arabidopsis pld knock-out mutants and PLD -silenced tomato cell-suspension cultures. pldα1 knock-out lines have reduced wounding-induced PA production, and the remainder is completely eliminated in a pldα1 / δ double knock-out line. Surprisingly, wounding-induced protein kinase activation, AtLOX2 gene expression and JA biosynthesis were not affected in these knock-out lines. Moreover, larvae of the Cabbage White butterfly ( Pieris rapae ) grew equally well on wild-type and the pld knock-out mutants. |
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Keywords: | jasmonic acid phosphatidic acid PLD |
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