首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Oral metagenomes from Native American Ancestors reveal distinct microbial lineages in the pre-contact era
Authors:Tanvi P Honap  Cara R Monroe  Sarah J Johnson  David K Jacobson  Christopher A Abin  Rita M Austin  Paul Sandberg  Marc Levine  Krithivasan Sankaranarayanan  Cecil M Lewis Jr
Institution:1. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA;2. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Department of Anthropology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Center for the Ethics of Indigenous Genomics Research (CEIGR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Contribution: Conceptualization (equal), Formal analysis (supporting), Funding acquisition (supporting), ​Investigation (lead), Project administration (equal), Supervision (supporting), Writing - original draft (supporting), Writing - review & editing (supporting);3. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Department of Anthropology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Contribution: Data curation (supporting), Formal analysis (equal), Visualization (equal), Writing - review & editing (supporting);4. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Department of Anthropology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Contribution: Formal analysis (supporting), Writing - review & editing (supporting);5. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Contribution: ​Investigation (supporting);6. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Department of Anthropology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Contribution: ​Investigation (supporting), Writing - review & editing (supporting);7. Department of Anthropology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Sam Noble Oklahoma Museum of Natural History, University of Oklahoma, 73072 Norman, Oklahoma, USA

Contribution: ​Investigation (supporting);8. Department of Anthropology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Sam Noble Oklahoma Museum of Natural History, University of Oklahoma, 73072 Norman, Oklahoma, USA

Contribution: Conceptualization (equal), Funding acquisition (supporting), Project administration (equal), Writing - original draft (supporting), Writing - review & editing (supporting);9. Laboratories of Molecular Anthropology and Microbiome Research (LMAMR), University of Oklahoma, 73072 Norman, Oklahoma, USA

Department of Microbiology and Plant Biology, University of Oklahoma, 73019 Norman, Oklahoma, USA

Contribution: Data curation (supporting), Formal analysis (equal), Supervision (supporting), Visualization (equal), Writing - review & editing (supporting)

Abstract:

Objectives

Limited studies have focused on how European contact and colonialism impacted Native American oral microbiomes, specifically, the diversity of commensal or opportunistically pathogenic oral microbes, which may be associated with oral diseases. Here, we studied the oral microbiomes of pre-contact Wichita Ancestors, in partnership with the Descendant community, The Wichita and Affiliated Tribes, Oklahoma, USA.

Materials and Methods

Skeletal remains of 28 Wichita Ancestors from 20 archeological sites (dating approximately to 1250–1450 CE) were paleopathologically assessed for presence of dental calculus and oral disease. DNA was extracted from calculus, and partial uracil deglycosylase-treated double-stranded DNA libraries were shotgun-sequenced using Illumina technology. DNA preservation was assessed, the microbial community was taxonomically profiled, and phylogenomic analyzes were conducted.

Results

Paleopathological analysis revealed signs of oral diseases such as caries and periodontitis. Calculus samples from 26 Ancestors yielded oral microbiomes with minimal extraneous contamination. Anaerolineaceae bacterium oral taxon 439 was found to be the most abundant bacterial species. Several Ancestors showed high abundance of bacteria typically associated with periodontitis such as Tannerella forsythia and Treponema denticola. Phylogenomic analyzes of Anaerolineaceae bacterium oral taxon 439 and T. forsythia revealed biogeographic structuring; strains present in the Wichita Ancestors clustered with strains from other pre-contact Native Americans and were distinct from European and/or post-contact American strains.

Discussion

We present the largest oral metagenome dataset from a pre-contact Native American population and demonstrate the presence of distinct lineages of oral microbes specific to the pre-contact Americas.
Keywords:ancient DNA  dental calculus  oral microbiome  oral pathogens
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号