| Affiliation: | 1. Centro de Ciências Exatas e Tecnológicas–CETEC, Universidade Federal do Recôncavo da Bahia, Rua Rui Barbosa, no710, 44.380-000 Cruz das Almas, Brazil;2. Metabolomics Research Group, Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo s/n, 40170-115 Salvador, Brazil;3. Programa de Pós-graduação em Ciências Agrárias, Centro de Ciências Agrárias, Ambientais e Biológicas–CCAAB, Universidade Federal do Recôncavo da Bahia, Rua Rui, Barbosa, no710, 44.380-000 Cruz das Almas, Brazil;4. Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas, Brazil Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas, 13083-871 Campinas, Brazil;5. Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas, Brazil;6. Brazilian Biosciences National Laboratory, Brazilian Center for Research in Energy and Materials, Rua Giuseppe Máximo Scolfaro 10.000, 13083-100 Campinas, São Paulo, Brazil |
| Abstract: | Pleurotus ostreatus is an edible fungus with high nutritional value that uses industrial and agricultural lignocellulosic residues as substrates for growth and reproduction. Understanding their growth metabolic dynamics on agro-industrial wastes would help to develop economically viable and eco-friendly biotechnological strategies for food production. Thus, we used UHPLC/MS/MS and GNPS as an innovative approach to investigate the chemical composition of two strains of P. ostreatus, coded as BH (Black Hirataki) and WH (White Hirataki), grown on sisal waste mixture (SW) supplemented with 20 % cocoa almond tegument (CAT) or 20 % of wheat bran (WB). Metabolite dereplication allowed the identification of 53 metabolites, which included glycerophospholipids, fatty acids, monoacylglycerols, steroids, carbohydrates, amino acids, and flavonoids. This is the first report of the identification of these compounds in P. ostreatus, except for the steroid ergosterol. Most of the metabolites described in this work possess potential biological activities, which support the nutraceutical properties of P. ostreatus. Thus, the results of this study provide essential leads to the understanding of white-rot fungi chemical plasticity aiming at developing alternative biotechnologies strategies for waste recycling. |
