Bacterial community structure in experimental methanogenic bioreactors and search for pathogenic clostridia as community members |
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Authors: | Dohrmann Anja B Baumert Susann Klingebiel Lars Weiland Peter Tebbe Christoph C |
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Institution: | (1) Institute of Biodiversity, Johann Heinrich von Th?nen-Institut (vTI), Bundesallee 50, 38116 Braunschweig, Germany;(2) Institute of Agricultural Technology and Biosystems Engineering, Johann Heinrich von Th?nen-Institut, Braunschweig, Germany; |
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Abstract: | Microbial conversion of organic waste or harvested plant material into biogas has become an attractive technology for energy
production. Biogas is produced in reactors under anaerobic conditions by a consortium of microorganisms which commonly include
bacteria of the genus Clostridium. Since the genus Clostridium also harbors some highly pathogenic members in its phylogenetic cluster I, there has been some concern that an unintended
growth of such pathogens might occur during the fermentation process. Therefore this study aimed to follow how process parameters
affect the diversity of Bacteria in general, and the diversity of Clostridium cluster I members in particular. The development of both communities was followed in model biogas reactors from start-up
during stable methanogenic conditions. The biogas reactors were run with either cattle or pig manures as substrates, and both
were operated at mesophilic and thermophilic conditions. The structural diversity was analyzed independent of cultivation
using a PCR-based detection of 16S rRNA genes and genetic profiling by single-strand conformation polymorphism (SSCP). Genetic
profiles indicated that both bacterial and clostridial communities evolved in parallel, and the community structures were
highly influenced by both substrate and temperature. Sequence analysis of 16S rRNA genes recovered from prominent bands from
SSCP profiles representing Clostridia detected no pathogenic species. Thus, this study gave no indication that pathogenic clostridia would be enriched as dominant
community members in biogas reactors fed with manure. |
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