Estimation of pyruvate decarboxylation in perfused rat skeletal muscle |
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| Authors: | P Schadewaldt U Münch M Prengel W Staib |
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| Institution: | 1. Department of Medical Chemistry, University of Helsinki, Helsinki, Finland;2. Department of General Microbiology, University of Helsinki, Helsinki, Finland;3. Max-Planck Institut für Molekulare Genetik, Berlin, West Germany |
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| Abstract: | A total of 46 E. coli strains showing mannose-resistant, P-blood-group independent hemagglutination of human erythrocytes were tested for binding to neuraminic acid. Nine of the strains completely lost their hemagglutination activity after the erythrocytes were treated with neuraminidase. To characterize the receptor structure, different neuraminic acid containing glycoproteins, their desialylated derivatives and neuraminyl oligosaccharides were tested for hemagglutination inhibition. These studies showed that the nine strains had binding specificity for alpha 2-3 linked neuraminic acid. |
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| Keywords: | PBS 10 mM sodium phosphate 0 15 M NaCl pH 7 1 |
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