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Microtubule-independent regulation of neurofilament interactions in vitro by neurofilament-bound ATPase activities |
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| Authors: | J.F. Leterrier P.A. Janmey J. Eyer |
| Affiliation: | a UMR CNRS 6187, Dpt. Neurosciences, P.B.S., Universite de Poitiers, 40 av. Recteur Pineau, 86022 Poitiers Cedex, France b Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA c Laboratoire de Neurobiologie et Transgénèse, UPRES-EA3143, INSERM IFR132, Equipe constituante de Génopole Ouest, Batiment Monteclair, CHU, 49033 Angers, France |
| Abstract: | Neurofilaments (NFs), the major neuronal intermediate filaments, form networks in vitro that mimic the axonal NF bundles. This report presents evidence for previously unknown regulation of the interactions between NFs by NF-associated ATPases. Two opposite effects on NF gelation in vitro occur at low and high ATP concentration. These findings support the hypothesis that NF bundles in situ are dynamic structures, and raise the possibility that ATP-hydrolyzing mechanoenzymes regulate their organization. |
| Keywords: | NFs, neurofilaments NFH, neurofilament high NFM, neurofilament medium NFL, neurofilament low MW, molecular weight subunits IFs, intermediate filaments MTs, microtubules KSP, lysine, serine, proline repeats IDPN, β,β&prime -imino-3-dipropionitrile |
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