Crystal structure of the E. coli tRNA aminoacyl stem isoacceptor RR-1660 at 2.0 Å resolution |
| |
Authors: | André Eichert Dominik Oberthür Jens P Fürste Volker A Erdmann |
| |
Institution: | a Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany b Institute of Biochemistry, Laboratory for Structural Biology of Infection and Inflammation, University of Lübeck, c/o DESY, Building 22a, 22603 Hamburg, Germany c Institute of Biochemistry and Food Chemistry, University of Hamburg, Notkestr. 85, c/o DESY, Building 22a, 22603 Hamburg, Germany |
| |
Abstract: | Due to the redundancy of the genetic code there exist six mRNA codons for arginine and several tRNAArg isoacceptors which translate these triplets to protein within the context of the mRNA. The tRNA identity elements assure the correct aminoacylation of the tRNA with the cognate amino acid by the aminoacyl-tRNA-synthetases. In tRNAArg, the identity elements consist of the anticodon, parts of the D-loop and the discriminator base. The minor groove of the acceptor stem interacts with the arginyl-tRNA-synthetase. We crystallized different Escherichia coli tRNAArg acceptor stem helices and solved the structure of the tRNAArg isoacceptor RR-1660 microhelix by X-ray structure analysis. The acceptor stem helix crystallizes in the space group P1 with the cell constants a = 26.28, b = 28.92, c = 29.00 Å, α = 105.74, β = 99.01, γ = 97.44° and two molecules per asymmetric unit. The RNA hydration pattern consists of 88 bound water molecules. Additionally, one glycerol molecule is bound within the interface of the two RNA molecules. |
| |
Keywords: | tRNA identity elements Acceptor stem microhelix tRNAArg/Arg-tRNA-synthetase system Crystal structure RNA hydration pattern |
本文献已被 ScienceDirect 等数据库收录! |
|