Diallyl sulfides in garlic activate both TRPA1 and TRPV1 |
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Authors: | Kanako Koizumi Masataka Narukawa Yuji Iitsuka Taiichiro Seki Tatsuo Watanabe |
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Institution: | a Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan b Global COE Program, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan c Department of Applied Life Sciences, Nihon University Graduate School of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan |
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Abstract: | We searched for novel agonists of TRP receptors especially for TRPA1 and TRPV1 in foods. We focused attention on garlic compounds, diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS). In TRPA1 or TRPV1 heterogeneously expressed CHO cells, all of those compounds increased Ca2+]i in concentration-dependent manner. The EC50 values of DADS and DATS were similar to that of allyl isothiocyanate (AITC) and that of DAS was 170-fold larger than that of AITC. Maximum responses of these sulfides were equal to that of AITC. The EC50 values of these compounds for TRPV1 were around 100 μM against that of capsaicin (CAP), 25.6 nM and maximum responses of garlic compounds were half to that of CAP. The Ca2+ responses were significantly suppressed by co-application of antagonist. We conclude that DAS, DADS, and DATS are agonist of both TRPA1 and TRPV1 but with high affinity for TRPA1. |
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Keywords: | TRP transient receptor potential TRPA1 transient receptor potential cation channel subfamily A member 1 TRPV1 transient receptor potential cation channel subfamily V member 1 DAS diallyl sulfide DADS diallyl disulfide DATS diallyl trisulfide AITC allyl isothiocyanate CAP capsaicin CNA cinnamaldehyde CPZ capsazepine [Ca2+]i intracellular Ca2+ concentration |
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