| 猪瘟和猪繁殖与呼吸综合征混合感染的快速检测及地域流行性分析 |
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| 引用本文： | 贾赟 张素芳 周斌 郑其升 赵玉军 陈溥言. 猪瘟和猪繁殖与呼吸综合征混合感染的快速检测及地域流行性分析[J]. Virologica Sinica, 2004, 19(5): 514-516 |
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| 作者姓名： | 贾赟 张素芳 周斌 郑其升 赵玉军 陈溥言 |
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| 作者单位： | [1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京210095 [2]沈阳农业大学畜牧兽医学院辽宁沈阳110161 |
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| 基金项目： | 国家高技术研究发展计划(863课题)资助(项目号:2001AA249012) |
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| 摘 要： | 呼吸道和繁殖障碍疾病是猪场最常见的两种疾病，严重影响猪群的健康和正常生产，已经给世界养猪业造成了很大的经济损失。而且这两种疾病的病原复杂多样，不同的猪场病因可能完全不同，从而给疾病的诊断与防治带来了极大的困难。多年的研究表明，呼吸道和繁殖障碍疾病的病原包
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| 关 键 词： | 猪瘟病毒 猪繁殖与呼吸综合征病毒 检测 流行性分析 |
Quick Detection for Porcine Reproductive and Respiratory Syndrome Virus and Classical Swine Fever Virus Mixed Infection and Regional EpidemiologyAnalysis |
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| JIAYun,,ZHANG Su-fang,ZHOU Bin,ZHENG Qi-sheng,ZHAO Yu-jun,CHEN Pu-yan. Quick Detection for Porcine Reproductive and Respiratory Syndrome Virus and Classical Swine Fever Virus Mixed Infection and Regional EpidemiologyAnalysis[J]. 中国病毒学(英文版), 2004, 19(5): 514-516 |
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| Authors: | JIAYun ZHANG Su-fang ZHOU Bin ZHENG Qi-sheng ZHAO Yu-jun CHEN Pu-yan |
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| Affiliation: | JIAYun1,2,ZHANG Su-fang1,ZHOU Bin1,ZHENG Qi-sheng1,ZHAO Yu-jun2,CHEN Pu-yan1** |
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| Abstract: | Using a pair of specific primers designed according to the relevant nucleotide sequence of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)VL2332 strain, Lv strain and Classical Swine Fever Virus(CSFV) C strain from GenBank, RT-PCR method was established to detect these two viruses at the same time. With this method, 87 tissue samples of 24 pig farms from Jiangsu, Shanghai and Fujian were tested. Among these samples 75 were PRRS positive, 56 were CSF positive, 53 were both PRRS and CSF positive, which amounts to 60.9 percent of all samples. Combined with regional epidemiology analysis, We concluded that samples from Fujian mainly mixed infected with PRRSV and CSFV, but samples from Jiansu simply infected with PRRSV. |
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| Keywords: | Porcine Reproductive and Respiratory Syndrome Virus Classical Swine Fever Virus Detecting analysis |
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