两种人乳腺癌裸鼠移植模型的建立

目的建立简便的人乳腺癌裸鼠移植模型,并探讨其部分生物学特性。方法采用雌激素受体阴性的MDA-MB-231和SK-BR-3人乳腺癌细胞株,分别接种于10只裸鼠左侧腋窝皮下,移植细胞总数为1×107/只。观察肿块生长情况,第42天处死荷瘤鼠,切除肿块作病理切片。结果 MDA-MB-231接种后第5d在接种部位可见结节,成瘤率为90%（9/10）,接种42 d肿瘤体积426.6±333.8,瘤重0.417±0.276,病理学检查为浸润性导管癌;SK-BR-3接种后第11天在接种部位可见结节,成瘤率为80%（8/10）,接种42 d肿瘤体积357.5±246,瘤重0.325±0.167,病理学检查为浸润性导管癌。结论该方法建立的人乳腺癌裸鼠移植模型,皮下移植方法简单,易于操作,成功率较高,肿瘤可部分保持人乳腺癌生物学特性,为研究人乳腺癌提供了重要工具。

Establishment of Two Human Breast Cancer Model in Nude Mice

Objective To establish proper model of human breast cancer in nude mice and study their biological features.Methods MDA-MB-231 and SK-BR-3 cells（ER-） were implanted into the left axilla of 10 nude mice.Tumor growth was observed,and the tumor-bearing mice were killed on day 42.The tumor masses were removed,and pathologically examined by the microscope.Results The MDA-MB-231 tumor tubercles could be formed on day 5.The tumor-take rate was 90%（9/10）,and average volume and weight of the tumor were（426.6±333.8）mm3 and（0.417±0.276） g reparatively.The SK-BR-3 tumor tubercles could be formed on day 11.The tumor-take rate was 80%（8/10）,and average volume and weight of the tumor were（357.5±246.4）mm3 and（0.325±0.167） g reparatively.All of the specimens were infiltrating ductal carcinoma.Conclusion We succeeded in establishing two human breast cancer model in nude mice,which reserved certain biological features of human breast cancer,therefore it may be an available model for further research of breast cancer.