葛根素预防雌激素缺乏性骨质疏松的机制探讨

目的观察葛根素对去卵巢大鼠股骨骨密度、颌骨骨密度和血清中肿瘤坏死因子（TNF-α）、C反应蛋白（CRP）及雌二醇（E2）水平的影响。方法 3月龄雌性SD大鼠随机分为假手术组（sham group）、单纯去势组（OVX group）、去势＋雌二醇治疗组（OVX-estrogen group）和去势＋葛根素治疗组（OVX-puerarin group）。给药3个月后测量各组大鼠股骨和颌骨骨密度,血清中TNF-α和CRP及雌二醇（E2）水平。结果 OVX-puerarin组较sham组体重明显增加（P〈0.01）;较OVX-estrogen组的TNF-α（P〈0.01）、CRP（P〈0.05）及E2（P〈0.01）水平显著下降;其下颌骨和股骨远端骨密度与OVX组、sham组之间无显著性差异,但其股骨近端骨密度较OVX-estrogen组明显增加（P〈0.05）。结论葛根素作为抗炎因子,能够显著降低TNF-α和CRP的水平,但并不会提高雌激素的水平;葛根素对卵巢切除所致的骨质疏松症有一定的治疗作用,但会明显提高机体体重。

Effect of Puerarin on the Osteoporosis Resulted from Ovariectomy in Rats

Objective To comparatively evaluate the therapeutic effect of puerarin on bone mineral density of the mandible and femur in ovariectomized rats,and to investigate the association between TNF-α,CRP and E2.Methods 40 female 3-month old SD rats were divided into 4 groups randomly： sham operation group（sham）,ovariectomized（OVX） group,ovariectomized group with injection of estrogen（OVX-estrogen） and ovariectomized group with injection of puerarin.After 3 months,TNF-α,CRP and E2 in the serum were tested.Meanwhile,the bone mineral density of the mandible and femur was measured.Results The body weight of OVX-puerarin group was signficantly higher than that of sham group（P 0.01）.Compared with OVX-estrogen group,the TNF-α,CRP and E2 serum level of OVX-puerarin group was significantly lower（respectively P 0.01,P 0.05,P 0.01）.As to mandible and distal femur bone mineral density,there were no significant difference among OVX-puerarin group,sham group and OVX group,but the proximal femur bone mineral density of OVX-puerarin group was significantly higher than that of OVX-estrogen group（P 0.05）.Conclusion As an anti-inflammatory factor,puerarin can significantly reduce the level of TNF-α and CRP in the serum,but can＇t promote the level of E2 in the serum in ovariectomized rats.Puerarin has some therapeutic effect on the ovariectomy-induced osteoporosis,but can significantly increase the body weight of ovariectomized rats.