SIVmac239感染恒河猴急性期回肠派氏淋巴结中淋巴细胞亚群的变化

目的分析SIVmac239感染早期中国恒河猴回肠派氏淋巴结淋巴细胞数量及亚群的变化,探讨这些变化与疾病进展的可能关系。方法以静脉注射SIVmac239制备恒河猴AIDS模型,对回肠派氏淋巴结进行CD4和CD8免疫组化标记,分离Peyer’s集合淋巴结淋巴细胞,分别标记CD3、CD4、CD8、CD28、CD95单克隆抗体,以流式细胞仪检测T细胞及其亚群的表达情况。结果 SIVmac239感染急性期中国恒河猴Peyer淋巴结中CD4＋/CD8＋比值持续下降,记忆性细胞比例升高,但Peyer淋巴结形态及CD4＋T细胞数量未见明显变化,CD8＋T细胞从第5天开始持续升高。结论 SIVmac239感染急性期,中国恒河猴回肠派氏淋巴结形态及CD4＋T细胞数量基本维持,向记忆性细胞的转化增加,但是CD4＋/CD8＋比值下降。

The Changes of Lymphocytes from Ileum Peyers Pacth from Rhesus Monkeys at the Earliest Period with SIVmac239 Infection

Objective To analyze the changes of lymphocytes from ileum Peyers pacth from Chinese rhesus monkeys at acute period with SIVmac239 infection.Methods 9 rhesus monkeys were used and 5 were infected with SIVmac239 via intravenous injection.The tissues or lymphocytes from ileum Peyers pacth lymph nodes from rhesus monkeys at acute period were collected and analyszed with CD4＋,CD8 immunohistochemistry and flow cytometry T-cell subsets labeling with CD3,CD4,CD8,CD28,CD95 monoclonal antibodies respectively.Results Obviously declined CD4＋/CD8＋ ratio and increased memory cells（CD4 ＋ CD8 ＋） ratio and CD8＋ T cells were detected in Peyers lymph nodes,but CD4 counts had no significant change in Peyers lymph nodes.Conclusion Declined CD4＋/CD8＋ ratio and increased memory cells（CD4 ＋ CD8 ＋） ratio were found in Peyers lymph nodes from rhesus macaques at acute period with SIVmac239 infection,however,the meaning and possible affection to disease need further study.