猴免疫缺陷病毒（SIV）感染恒河猴血液学和血液生化的动态变化

目的探讨SIVmac239感染中国恒河猴的血液学和血清生物化学的变化,研究艾滋病的发生机制。方法 20只恒河猴感染SIVmac239后分别在感染前和感染后2、6、9、12、15和18个月在猴空腹状态用盐酸氯胺酮麻醉下静脉釆血。抗凝血用全自动血液细胞分析仪检测血液常规,血清用全自动生化分析仪检测生化指标。结果SIV感染猴红白细胞计数呈逐渐的减少。淋巴细胞计数有较明显减/增的波动。血清酶类ALT和AST没有明显的上升,但在15月时均有所下降P〈0.05～P〈0.01。LDH持续降低,原因未明。CK则逐月上升,至12月时达高峰,后又有所下降。血尿素氮逐月上升,并在6～18个月时P〈0.01。总蛋白量上升,白蛋白逐月降低,球蛋白升高,白蛋白/球蛋白比率倒置。SIV感染猴2个月后总胆固醇、甘油三脂、低密度脂蛋白和高密度脂蛋白均呈上升,至12月时略有下降,但到18月又上升。血糖（GLU）在SIV感染后逐月上升,后期上升更为明显,这可能与胰岛的损害有关。结论 SIVmac239感染猴后,通过SIV侵入相关的器官病变和体液调节失衡导致血液学和血液生物化学产生异常,从而提供有关猴艾滋病的一些基础资料,这将对研究人的艾滋病也许有所帮助。

Dynamic Changes of Haematological and Blood Biochemical Values in SIV-infected Rhesus Monkeys

Objective The aim is to explore the hematological and the blood biochemical values in SIV infected monkeys for the investigation of pathogenesis of SIV/HIV.Methods 20 Chinese rhesus monkeys were inoculated with SIVmac 239 intravenously,and the blood samples were taken from monkeys in 2,6,9,12,15 and l8 months under anesthesia with ketamine hydrochloride.The anticoaglutin blood was used for blood routine by Beckman Coulter Ac.T5 diff,and the serum was analyzed for biochemical elements by the use of Hitachi 7020 automatic analyzer.Result The total count of erythrocyte and leukocyte revealed to be decreased slowly but the count of lymphocyte fluctuated up and down between 52.1±13.1% and 32.8 ±14.2%.ALT and AST did not appeared to be increased,but at the time of 15 months they increased P 0.05 and P 0.01.LDH decreased but the cause is unknown.CK increased steadily to the summit in12 months then it fell down.The BUN increased step by step monthly to 6~18 months P 0.01.The total protein increased but the albumin decreased,the ratio of A/G was inversed.Total cholesterol,triglyceride,HDL and LDL increased but they decreased in 12 months.Blood glucose increased monthly,it may be related to the damage of pancreatic islet in SIV infected monkeys.Conclusion By the use of SIV infected monkeys more organs suffered with pathological changes and dysfunction of body liquid,some haematological and blood biochemical values appeared to be abnormal,by which it would be presented some useful data for the investigation of pathogenesis of SIV/HIV.