Determination of diarrhetic shellfish toxins in various dinoflagellate species |
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Authors: | Jong-Soo Lee Teruo Igarashi Santiago Fraga Einal Dahl Peter Hovgaard Takeshi Yasumoto |
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Affiliation: | 1. Department of Food Chemistry, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Sendai, 980, Japan 2. Miyagi Prefectural Kesennuma Fisheries Experimental Station, Hajikami, Kesennuma, Miyagi, 988-02, Japan 3. Instituto Espanol De Oceanografia, Centro Costero de Vigo, Cabo Estay-Canido, Apartado 1.552, 36080, Vigo, Spain 4. Flordevigen Biological Station, N-4800, Arendal, Norway 5. Sogn og Fjordane Distriktshogskule, N-5800, Norway
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Abstract: | Sixteen species of unialgal samples of dinoflagellate, either wild or cultured, were tested for production of diarrhetic shellfish toxins such as okadaic acid (OA), dinophysistoxin-1 (DTX1), and pectenotoxins (PTXs). Determination of micro-quantities of the toxins was facilitated by fluorometry and UV HPLC. Seven Dinophysis species were confirmed to produce either OA or DTX1, or both. Toxin content and composition varied regionally and seasonally. Intraspecies variation was also observed among cultured strains of Prorocentrum lima. PTX2 was the only toxin detected among PTX family, and D. fortii was the only species to contain this toxin. author for correspondence |
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Keywords: | diarrhetic shellfish poisoning fluorometric HPLC determination Dinophysis spp. Prorocentrum lima |
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