The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria |
| |
Authors: | T Kubo M P Yamamoto T Mikami |
| |
Institution: | (1) Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060–8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP |
| |
Abstract: | We have found that a gene coding for NADH dehydrogenase subunit 4L and a presumed gene, orf25, are linked and co-transcribed with each other in sugar beet mitochondria. Ten and twelve C-to-U editing events were observed
in the mRNAs of nad4L and orf25, respectively; the amino-acid sequence specified after editing is better-conserved in comparison with the homologues of other
organisms. It is interesting to note that the translation initiation codon of nad4L is created by editing. The conservation of the nad4L-orf25 linkage was examined by PCR-amplification of the intergenic region. We obtained successful PCR products from five dicots
(spinach, apple, snapdragon, petunia and tobacco) and two monocots (tulip and pineapple), but not in two poaceous plants,
rice and maize. The intergenic region, when present, was found to be well-conserved in its sequence, suggesting a monophyletic
origin of this linkage. Our result, together with previous reports of Arabidopsis and four poaceous species, favour the argument that the nad4L-orf25 linkage is conserved throughout angiosperms except in the Poaceae.
Received: 12 April 1999 / Accepted: 22 June 1999 |
| |
Keywords: | Nad4L-orf25 linkage RNA editing Sugar beet Mitochondria |
本文献已被 SpringerLink 等数据库收录! |
|