Simultaneous determination of fatty, dicarboxylic and amino acids based on derivatization with isobutyl chloroformate followed by gas chromatography-positive ion chemical ionization mass spectrometry |
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Authors: | Sobolevsky Tim G Revelsky Alexander I Revelsky Igor A Miller Barbara Oriedo Vincent |
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Institution: | Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1 Building 3, 119992, Moscow, Russia. sobolevsky@environment.chem.msu.ru |
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Abstract: | Gas chromatography-mass spectrometry (GC-MS) with positive ion chemical ionization (PICI) using isobutane as reagent gas was applied for analysis of isobutoxycarbonyl/isobutyl derivatives of 13 fatty, 6 dicarboxylic and 13 amino acids in a single run. For all investigated compounds (except several amino acids) the quasimolecular ions MH](+) were registered. Asparagine underwent fragmentation via decarboxylation followed by elimination of OC(4)H(9) (M-117](+)), whereas serine and tyrosine produced the cluster ions M+C(4)H(9)OCO](+). Estimated detection limits were 6-250 pg in the total ion current (TIC) mode and 3-10 times lower using the selected-ion monitoring (SIM) mode. |
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