Isolation and characterization of the nuclei fromSaccharomyces cerevisiae |
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| Authors: | Kazuo Kamimura Eiko Tsuchiya Tokichi Miyakawa Sakuzo Fuki Aiko Hirata |
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| Affiliation: | (1) Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Sendamachi 3, Naka-ku, 730 Hiroshima, Japan;(2) Institute of Applied Microbiology, The University of Tokyo, Yayoi 1, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | We isolated highly intact nuclei from cells of a haploid strain ofSaccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei. |
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