Oligodeoxynucleotide stability in subcellular extracts and culture media |
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| Authors: | E Wickstrom |
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| Institution: | 1. Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, Rio de Janeiro 21941-902, Brazil;2. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Avenida Dr. Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceará 60714-903, Brazil;3. Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Prof. Lineu Prestes, 1524, Cidade Universitária “Armando Salles Oliveira”, São Paulo, São Paulo 05508-900, Brazil;4. Instituto Nacional de Ciência e Tecnologia em Biologia e Bioimagem, Avenida Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, Rio de Janeiro 21941-902, Brazil |
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| Abstract: | Oligodeoxynucleotide degradation was studied in four systems in order to assess the importance of degradation in hybridization arrest experiments dependent on oligodeoxynucleotides complementary to mRNA sequences. Oligodeoxynucleotides were not detectably degraded over 2 h at 37 degrees C in rabbit reticulocyte lysate or Dulbecco's modified essential medium with 5% fetal calf serum, but were degraded over 2 h in HeLa cell postmitochondrial cytoplasmic extract, and were degraded within 15 min in bovine calf serum. |
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