A Simple and Rapid Method Enhancing of Lipoxygenase-1 to Lipoxygenase-2+Lipoxygenase-3 Isoenzyme Activity Ratio in Soybean Meal Extracts |
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| Authors: | Ágnes Sz Németh Béla Szajáni Judit Sz Márczy Mária L Simon |
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| Institution: | (1) Research Institute of Chemical and Process Engineering, Pannon University of Agricultural Sciences, P.O. Box 125, Veszprém, Hungary, H-8201;(2) COVENT Industrial Venture Capital Investment Co., Budapest, Hungary;(3) Department of Biochemistry, Attila József University, Szeged, Hungary |
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| Abstract: | Lipoxygenase-2 and lipoxygenase-3 isoenzymes can be eliminated from soybean (Glycine max) meal extract by a simple selective heat treatment. The optimum conditions were: 70 °C, for 5 min at pH 5.2 with an ionic strength of 0.05. The activity ratio of lipoxygenase-1 to lipoxygenase-2 + lipoxygenase-3 was enhanced from 5 to about 21. The resulting enzyme can be used immediately for hydroperoxidation or frozen without loss of activity. |
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