首页 | 本学科首页   官方微博 | 高级检索  
   检索      


anota: Analysis of differential translation in genome-wide studies
Authors:Larsson Ola  Sonenberg Nahum  Nadon Robert
Institution:Department of Biochemistry, McGill University, Montreal, Quebec, Canada. ola.larsson@ki.se
Abstract:Translational control of gene expression has emerged as a major mechanism that regulates many biological processes and shows dysregulation in human diseases including cancer. When studying differential translation, levels of both actively translating mRNAs and total cytosolic mRNAs are obtained where the latter is used to correct for a possible contribution of differential cytosolic mRNA levels to the observed differential levels of actively translated mRNAs. We have recently shown that analysis of partial variance (APV) corrects for cytosolic mRNA levels more effectively than the commonly applied log ratio approach. APV provides a high degree of specificity and sensitivity for detecting biologically meaningful translation changes, especially when combined with a variance shrinkage method for estimating random error. Here we describe the anota (analysis of translational activity) R-package which implements APV, allows scrutiny of associated statistical assumptions and provides biologically motivated filters for analysis of genome wide datasets. Although the package was developed for analysis of differential translation in polysome microarray or ribosome-profiling datasets, any high-dimensional data that result in paired controls, such as RNP immunoprecipitation-microarray (RIP-CHIP) datasets, can be successfully analyzed with anota. AVAILABILITY: The anota Bioconductor package, www.bioconductor.org.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号