Synchrotron-excited time-resolved fluorescence spectroscopy of adenosine,protonated adenosine and 6N, 6N-dimethyladenosine in aqueous solution at room temperature |
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| Authors: | J -P Ballini M Daniels P Vigny |
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| Institution: | (1) Laboratoire de Physique et de Chimie Biomoléculaire C.N.R.S. (UA198) Institut Curie 11, rue Pierre et Marie Curie, F-75231 Paris Cedex 05, France;(2) Université Pierre et Marie Curie, (Paris VI);(3) Chemistry Department, Oregon State University, 97331 Corvallis, OR, USA;(4) Radiation Center, Oregon State University, 97331 Corvallis, OR, USA;(5) Laboratoire d'Utilisation du Rayonnement Electromagnétique (L.U.R.E.), C.N.R.S., Bât. 209 C, F-91405 Orsay Cedex, France;(6) Université Paris-Sud, Bât. 209 C, F-91405 Orsay Cedex, France |
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| Abstract: | The fluorescence behavior of adenosine in neutral solution has been studied by time-resolved spectroscopy using synchrotron excitation and timecorrelated single photon counting, and by decay time measurements. Three emissions have been identified and correlated with three excitation spectra. The assignment of these transitions has been made by comparison with similar measurements on 6N, 6N-dimethyladenosine (6 DMA), and on adenosine in acid solution (ADO H+). It is proposed that two of the transitions of adenosine which correlate with 6DMA originate from coplanar and orthogonal rotational conformers of the amino group. The other transition, correlating with ADO H+ may originate either from the 3H-imino tautomer, or from a differently solvated rotational conformer.A partial presentation of this work has been made at the Second Congress of the European Society for Photobiology Padova, Italy, 6–10 September 1987 |
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| Keywords: | Adenosine time-resolved fluorescence spectroscopy fluorescence excitation decay time of fluorescence dual fluorescence |
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