Peptide presentation by bacteriophage P4 |
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Authors: | Bj/orn H Lindqvist Soheil Naderi |
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Institution: | Institute of Biology and The Biotechnology Centre of Oslo, University of Oslo, 0371 Oslo, Norway |
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Abstract: | Abstract: This article focuses on bacteriophage P4 as a potential peptide display phage by exploring the possibility of using the P4 capsid decoration component, Psu, as a peptide carrier protein. Psu is non-essential for P4 growth but it enhances the stability of the P4 capsid by binding to its exterior. We have constructed a unique Sac I cloning site in the beginning of the psu gene. This site changes the third amino acid of Psu from Ser to Leu. This substitution does not destroy the binding of Psu to the P4 capsid. A synthetic oligonucleotide encoding a 10-amino acid peptide whose sequence is part of the human p62c-myc protein, has been inserted into the Sac I site. The Psuc-myc shows full capsid binding activity and reacts with monoclonal antibodies directed against the c-myc peptide. These results pave the way for the further development of a peptide display system based on bacteriophage p4. |
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Keywords: | Phage P4 Phage display Peptide C-myc |
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