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Histochemical staining of cadmium thiolate clusters in livers of rats treated chronically with cadmium |
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| Authors: | A F W Morselt N M van Straalen |
| Institution: | (1) Laboratory for Histology and Cell Biology, University of Amsterdam, Academic Medical Center, Meibergdreef 15, NL-1105 AZ Amsterdam, The Netherlands;(2) Department of Biology, Free University of Amsterdam, P.O. Box 7161, NL-1007MC Amsterdam, The Netherlands |
| Abstract: | Summary In livers of rats exposed to varying doses of CdCl2 80–90% of the cadmium content present in the fresh tissue is retained if these livers are fixed with a neutral or acid formalin fixative.Cadmium assays during different stages of the staining procedure for protein bound disulphides show the ability of this staining to demonstrate cadmium thiolate clusters next to disulphides. The methods described may also be useful in gaining more insight in the mechanism involved in fixation and staining procedure of some other metals. |
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