Conversion of fatty acids by Bacillus sphaericus-like organisms |
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Authors: | Kuo Tsung Min Nakamura Lawrence K Lanser Alan C |
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Institution: | (1) Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA, US |
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Abstract: | Bacillus sphaericus species are mesophilic round-spored organisms that readily utilize fatty acid-based surfactants during growth, but their
ability to modify fatty acids is unknown. Among 57 B. sphaericus-like strains tested for fatty acid transformation activity in Wallen fermentation (WF) medium, ten converted oleic acid to
a new product determined by gas chromatography – mass spectrometry (GC-MS) to be 10-ketostearic acid (10-KSA). Additionally,
a few other strains converted ricinoleic acid and linoleic acid to new products that remain to be characterized. Unlike most
microbial hydrations of oleic acid, which produce a mixture of 10-KSA and 10-hydroxystearic acid, the conversion of oleic
acid by B. sphaericus strains was unique in that 10-KSA was the sole reaction product. By replacing dextrose with sodium pyruvate in WF and adjusting
to pH 6.5, conversion of oleic acid to 10-KSA by strain NRRL NRS-732 was improved from about 11% to more than 60%. Using the
defined optimal conditions, the conversion reaction was scaled up in a stirred-batch reactor by using technical-grade oleic
acid as substrate. This is the first report on the characterization of fatty acid conversions by B. sphaericus species.
Received: 17 December 2001 / Accepted: 17 January 2002 |
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