| UV-C照射诱导体外血管平滑肌细胞凋亡模型的建立 |
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| 作者姓名： | Li XD Li J |
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| 作者单位： | 1. 广州军区医学高等专科学校内科教研室,广州,510315
2. 第一军医大学组织胚胎学教研室,广州,510515,234 |
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| 摘 要： | 应用常规细胞培养超净台紫外消毒灯（２２０Ｗ,２２０Ｖ,５０Ｈｚ）发射的ＵＶ－Ｃ波段的紫外光源（２５４ｎｍ）,垂直照射距离其１０ｃｍ处的大鼠主动脉平滑肌细胞,发现经照射后细胞出现典型的凋亡形态学改变,如细胞变圆,染色质浓缩,细胞膜出泡,出现凋亡小体等;细胞面积,核面积及核／胞面积比均显著降低;且提取细胞ＤＮＡ的琼脂糖凝胶电泳呈现梯状图谱。从形态学和生化指标方面证明了ＵＶ－Ｃ照射可诱导体外血管ＳＭＣｓ
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| 关 键 词： | 平滑肌细胞 细胞凋亡 紫外线 动脉粥样硬化 |
Establishment of an apoptosis model of vascular smooth muscle cells induced by ultraviolet-C radiation in vitro |
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| Li XD,Li J.Establishment of an apoptosis model of vascular smooth muscle cells induced by ultraviolet-C radiation in vitro[J].Acta Physiologica Sinica,1999,0(2):234-239. |
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| Authors: | Li X D Li J |
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| Institution: | Department of Internal Medicine of Guangzhou Medical College, PLA, Guangzhou 510315. |
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| Abstract: | After a 10min exposure to 254 nm UVC radiation, aortic SMCs of rat were found to undergo apoptosis, characterized by cell shrinkage, condensation of cytoplasm, membrane blebbing, appearance of apoptotic body and DNA ladder in the agarose gel. Therefore, UVC radiation can be used to generate a useful model for the investigation of the molecular regulatory mechanisms underlying SMCs apoptosis. |
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| Keywords: | smooth muscle cells apoptosis ultraviolet atherosclerosis |
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