Acetate utilization by a methylotrophic bacterium |
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Authors: | Edward Bellion Yu Sam Kim |
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Affiliation: | (1) Department of Chemistry, University of Texas at Arlington, 76019 Arlington, Texas, USA |
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Abstract: | The mode of acetate ultilization in the Gram-negative diplococcoid bacterium PAR was investigated. This organism uses the isocitrate lyase-negative serine pathway during growth on C1-compounds and was found to lack isocitrate lyase on acetate growth also. Enzyme assays revealed the absence of the glycerate and hydroxyaspartate pathways for the metabolism of C2-compounds. Pulse-labeling experiments indicated the rapid formation of glycine, glutamate, and malate. The rapid formation of malate and the presence of malate synthase suggest that glyoxylate is an intermediate formed from acetate by a means other than the conventional isocitrate cleavage reaction. |
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