Sulfonylurea enhances insulin-induced acetyl coenzyme A carboxylase activity in rat adipocytes. |
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| Authors: | M Matsuda K Kaku M Aoki H Inoue T Kaneko |
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| Institution: | Third Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan. |
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| Abstract: | The effect of sulfonylurea on the activity of acetyl-coenzyme A carboxylase, a rate limiting enzyme of lipogenesis, was investigated using isolated rat adipocytes. Insulin significantly increased the enzyme activity by 170% of the control level, while glucagon and epinephrine decreased the activity of the enzyme by 53% and 64% of the control, respectively. In the presence of tolbutamide (10(-3) M) or glibenclamide (10(-6) M), a significant potentiation of insulin action was found in adipocytes. In addition, sulfonylurea restored the activity of acetyl-CoA carboxylase reduced by glucagon or epinephrine to the control level. Sulfonylurea enhancement of the acetyl-CoA carboxylase activity may offer one possible explanation for a mechanism of antilipolytic action of the drug in adipocytes. |
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