Role of plastidial acyl-acyl carrier protein: Glycerol 3-phosphate acyltransferase and acyl-acyl carrier protein hydrolase in channelling the acyl flux through the prokaryotic and eukaryotic pathway |
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| Authors: | Imma Löhden Margrit Frentzen |
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| Affiliation: | (1) Department of Biochemistry, Royal Holloway and Bedford New College, University of London, TW20 0EX Egham, Surrey, UK;(2) M.R.C. Laboratory of Molecular Biology, Hills Road, CB2 2OH Cambridge, UK;(3) Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RA Oxford, UK;(4) Oxford Oligosaccharide Group, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU Oxford, UK;(5) Present address: Celltech Ltd., 216 Bath Road, SL1 4EN Slough, UK |
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| Abstract: | Monoclonal antibodies raised against extracts of the rachis abscission zone of Sambucus nigra L. were selected for high reactivity towards abscission-zone proteins. One antibody (YZ1/2.23) has been shown to cross-react, by both indirect and competition enzyme-linked immunosorbent assay and by Western blotting, with a number of plant enzymes including horseradish peroxidase, rice  -glucosidase, almond  -glucosidase and the lectins from Phaseolus vulgaris and Erythrina cristagalli.The major N-linked oligosaccharide isolated from horseradish peroxidase has the sequence Man 3(Man6)(Xyl2)Man4GlcNAc4(Fuc3) GlcNAc. This oligosaccharide was found to be a potent inhibitor of the binding of YZ1/2.23 to the intact glycoprotein. The common determinant is therefore contained within this structure.Abbreviations ELISA enzyme-linked immunosorbent assay - Fuc fucose - GlcNAc N-acetylglucosamine - HRP horseradish peroxidase - Ig immunoglobulin - Man mannose - SDS-PAGE Sodium dodecyl sulphate-polyacrylamide gel electrophoresis - Xyl xylose |
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| Keywords: | Abscission Glycoprotein Monoclonal antibody N-linked oligosaccharide Sambucus |
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