Detection of citrinin in ochratoxin A-containing products by a new HPLC method |
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Authors: | U Meister |
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Institution: | (1) IGV Institut für Getreideverarbeitung GmbH, Arthur-Scheunert-Allee 40-41, D-14558 Bergholz-Rehbrücke |
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Abstract: | A new method for citrinin was developed and validated, which is based on solid phase extraction with polyamide columns and
HPLC with fluorescence detection. Sufficient skill with the method given, precise results, i.e. variation coefficients <10%,
will be achieved. The mean recovery rates were in the range 74 – 90%. The detection limits of the method determined according
to DIN 32645, at good precision, were 1 μg/kg for wheat, rye, barley, maize, and oats. The analysis of several samples containing
ochratoxin A (OTA) showed that citrinin is present in brans, wheatings and shorts containing a higher ratio of the outer layers
of the grain kernel; both OTA and citrinin were found in in cocoa shells and raisins. Citrinin was detected in 14 OTA-containing
samples (1–8 μg/kg). Furthermore, it was demonstrated that citrinin also can be determined in red mold rice according to the
new method.
Presented at the 25th Mykotoxin Workshop in Giessen, Germany, May 19–21, 2003 |
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Keywords: | mycotoxin citrinin analysis HPLC cereals |
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