An in vitro urinary tract catheter system to investigate biofilm development in catheter-associated urinary tract infections |
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| Affiliation: | 1. Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium;2. Laboratory of Medical Microbiology (LMM), University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium;3. Intensive Care Unit, Antwerp University Hospital, University of Antwerp, Wilrijkstraat 10, 2650 Edegem, Belgium;1. Department of Biomolecular Sciences, University of Urbino, 61029 Urbino, PU, Italy;2. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, PD, Italy;1. Laboratory of Microbiology and Immunology, School of Chinese and Western Integrative Medicine, Anhui University of Chinese Medicine, Hefei 230038, China;2. Laboratory of Biochemistry and Molecular Biology, School of Chinese and Western Integrative Medicine, Anhui University of Chinese Medicine, Hefei 230038, China;3. Integrated Traditional Chinese Medicine and Western Medicine, School of Acupuncture and Moxibustion & Fractures, Anhui University of Chinese Medicine, Hefei 230038, China |
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| Abstract: | Biofilm development in urinary tract catheters is an often underestimated problem. However, this form of infection leads to high mortality rates and causes significant costs in health care. Therefore, it is important to analyze these biofilms and establish avoiding strategies. In this study a continuous flow-through system for the cultivation of biofilms under catheter-associated urinary tract infection conditions was established and validated. The in vitro urinary tract catheter system implies the composition of urine (artificial urine medium), the mean volume of urine of adults (1 mL min–1), the frequently used silicone catheter (foley silicon catheter) as well as the infection with uropathogenic microorganisms like Pseudomonas aeruginosa. Three clinical isolates from urine of catheterized patients were chosen due to their ability to form biofilms, their mobility and their cell surface hydrophobicity. As reference strain P. aeruginosa PA14 has been used. Characteristic parameters as biofilm thickness, specific biofilm growth rate and substrate consumption were observed. Biofilm thicknesses varied from 105 ± 16 μm up to 246 ± 67 μm for the different isolates. The specific biofilm growth rate could be determined with a non invasive optical biomass sensor. This sensor allows online monitoring of the biofilm growth in the progress of the cultivation. |
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