| 人钠/碘同向转运体基因cDNA的克隆与序列测定 |
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| 引用本文： | 陈立波,朱瑞森,黄梅,黄芳,费俭,郭礼和.人钠/碘同向转运体基因cDNA的克隆与序列测定[J].生物技术通讯,2003,14(6):506-508. |
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| 作者姓名： | 陈立波 朱瑞森 黄梅 黄芳 费俭 郭礼和 |
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| 作者单位： | 1. 上海交通大学,附属第六人民医院,上海,200233
2. 中国科学院,生物化学与细胞生物学研究所,上海,200031 |
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| 摘 要： | 为研究人钠／碘同向转运体(hNIS)的生物学性能和用于肿瘤放射性碘治疗的可能性，运用反转录聚合酶链反应(RT—PCR)从人甲状腺组织总RNA中扩增出hNIS基因cDNA序列，将其克隆至pUCm-T载体中。序列分析证实克隆片段与献报道的hNIS基因cDNA序列完全一致，说明已成功克隆到hNIS基因cDNA。
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| 关 键 词： | 人钠／碘同向转运体基因 cDNA 克隆 序列测定 |
| 文章编号： | 1009-0002(2003)06-0506-03 |
| 修稿时间： | 2003年4月28日 |
Cloning and sequencing of human sodium/iodide symporter |
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| CHEN Li-bo,ZHU Rui-sen,HUANG Mei,HUANG Fang,FEI Jian,GUO Li-he.Cloning and sequencing of human sodium/iodide symporter[J].Letters in Biotechnology,2003,14(6):506-508. |
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| Authors: | CHEN Li-bo ZHU Rui-sen HUANG Mei HUANG Fang FEI Jian GUO Li-he |
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| Abstract: | For studying biological actions and potential utility as a radioiodide treatment for undifferentiated thyroid carcinoma and nonthyroid carcinoma, human sodium/iodide symporter (hNIS) gene cDNA was amplified with total RNA from human thyroid tissue by RT-PCR. The recombinant plasmid pUCm-T-NIS was constructed by inserting the hNIS cDNA into cloning vector pUCm-T and transformed into E.coli XL-B. Screen the positive clone which contains the cDNA of hNIS. The cloned hNIS cDNA sequence is identical to published sequence. |
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| Keywords: | human sodium/iodide symporter cDNA cloning sequencing |
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