|
|||||
|
|
Progestins stimulate the differentiation of 3T3-L1 preadipocytes |
|
| Authors: | Cristina M Rondinone Michael E Baker and David Rodbard |
| Institution: | 1 Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD, U.S.A. 2 Department of Medicine, 0623B, University of California at San Diego, La Jolla, CA 92093-0623, USA 3 Division of Computer Research and Technology, National Institutes of Health, Bethesda, MD 20892, U.S.A. |
| Abstract: | The effect of progesterone on the differentiation of the 3T3-L1 preadipocytes was investigated and compared with other sex steroids (estradiol and testosterone), with cortisol, with the synthetic progestin R5020 and with the progestin/glucocorticoid antagonist RU38486. At 10−8 M, progesterone stimulated the activity of glycerol-3-phosphate dehydrogenase and triglyceride deposition. Progesterone, R5020, cortisol, and RU38486 increased triglycerides about 2-fold at 10−7 M. Only minimal effects were observed with testosterone and estradiol even at 10−6 M. When the cells were cultured in presence of 10−5 M metyrapone the effect of progesterone was unchanged, suggesting that the progesterone was not metabolized to a glucocorticoid. Progesterone, R5020 and RU38486 competed efficiently with 3H]dexamethasone for the glucocorticoid receptor in 3T3-L1 cytosol. These results indicate a significant, reproducible dose-dependent effect of progestins on differentiation of the preadipocytes, which appears to be mediated via the glucocorticoid receptor. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |