The immunofluorescent demonstration of cathepsin B1 in tissue sections |
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Authors: | B. Sylvén O. Snellman |
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Affiliation: | (1) The Cancer Research Division of Radiumhemmet, Karolinska Institute, Stockholm, Sweden;(2) the Cancer Research Division, Institute of Pathology, University of Zürich, Zürich, Switzerland;(3) Abteilung für Krebsforschung, Institut für Pathologische Anatomie, Birchstraße 95, CH-8050 Zürich, Switzerland |
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Abstract: | Summary A direct immunohistochemical method of high specificity is presented for the demonstration of sites of cathepsin B1. Antisera were raised in rabbits against pure enzyme preparations. The specific antibodies were subsequently isolated and labelled according to methods previously published. Sections from tissues specially rich in this enzyme like liver, kidney, intestinal epithelia and the active macrophages of talcum granulomas were studied. The results suggest a localization of this enzyme mainly to lysosomal organelles and phagocytic vacuoles, which are known from chemical data to contain this enzyme. The prerequisite for a positive tissue reaction is that the enzyme must be present in a form available for antigen-antibody interaction. |
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