Isolation and characterization of a 23 kDa protein essential for photosynthetic oxygen evolution |
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Authors: | Christer Jansson Hans -Erik Åkerlund Bertil Andersson |
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Institution: | (1) Department of Biochemistry, University of Lund, P.O. Box 740, 8-220 07 Lund, Sweden |
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Abstract: | A 23 kDa protein has recently been demonstrated to participate in photosynthetic oxygen evolution by reconstitution experiments on inside-out thylakoid vesicles (Åkerlund H-E, Jansson C and Andersson B (1982) Biochim Biophys Acta 681:1–10). Here we describe the isolation of the 23 kDa protein from a spinach chloroplast extract using ion-exchange chromatography. The protein was obtained in a yield of 25% and with less than 1% of contaminating proteins. The ability of the protein to stimulate oxygen evolution in inside-out thylakoids was preserved throughout the various fractionation steps. The isolated protein was highly water soluble and appeared as a monomer. Its isoelectric point was at pH=7.3. The amino acid composition showed a high content of polar amino acids, resulting in a polarity index of 49%. The isolated protein lacked metals and other prosthetic groups. Its function as a catalytic or regulating subunit in the oxygen evolving complex is discussed.Abbreviations kDa
kiloDalton
- PAGE
polyacrylamide gel electrophoresis |
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Keywords: | electron transport inside-out thylakoids oxygen evolution photosynthesis thykoid polypeptides |
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