Diarrheal and Environmental Isolates of Aeromonas spp. Produce a Toxin Similar to Shiga-Like Toxin 1 |
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| Authors: | Quazi Manjurul Haque Akira Sugiyama Yoshito Iwade Yutaka Midorikawa Toru Yamauchi |
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| Institution: | (1) Department of Public Health, School of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie-Ken 514, Japan , JP;(2) Section of Microbiology, Mie Prefectural Institute of Public Health, 3-446-34, Sakurabashi, Tsu, Mie-Ken 514, Japan , JP;(3) Department of Clinical Nutrition, Suzuka University of Medical Science and Technology, 1001-01 Kishioka-cho, Suzuka, Mie-Ken 510-02, Japan , JP |
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| Abstract: | Diarrheal and environmental isolates of 39 strains of Aeromonas spp. were studied for detection of virulence factors. Although these 39 strains did not produce either heat-labile or heat-stable
enterotoxins, culture filtrates of 31 strains produced cytopathic effects on Vero cells. Among these, culture filtrates of
three strains of Aeromonas hydrophila and one strain of Aeromonas caviae could be neutralized by Escherichia coli O157:H7 Shiga-like toxin 1 antiserum. A single band of plasmid DNA of 2.14 kbp was isolated from these strains of Aeromonas spp. and E. coli O157:H7, which could be amplified by the polymerase chain reaction (PCR), employing oligonucleotide primers from the Shiga-like
toxin 1 (SLT1) gene of E. coli O157:H7. E. coli HB 101 cells when transformed with the same plasmid showed cytopathic effects on Vero cells, which indicates that the SLT
1 homolog gene(s) of Aeromonas spp. is plasmid encoded. These results suggest that Aeromonas spp. may also produce Shiga-like toxin 1, or at least a cytotoxin with some homology with the Shiga-like toxin 1 of E. coli O157:H7. |
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