首页 | 本学科首页   官方微博 | 高级检索  
     


Catalysis of regioselective reduction of NAD+ by ruthenium(II) arene complexes under biologically relevant conditions
Authors:Yaw Kai Yan  Michael Melchart  Abraha Habtemariam  Anna F. A. Peacock  Peter J. Sadler
Affiliation:(1) School of Chemistry, University of Edinburgh, West Mains Road, EH9 3JJ Edinburgh, UK;(2) Present address: Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Republic of Singapore
Abstract:Ruthenium(II) arene anticancer complexes [(η 6-arene)Ru(en)Cl]PF6 (arene is hexamethylbenzene, p-cymene, indan; en is ethylenediamine) can catalyse regioselective reduction of NAD+ by formate in water to form 1,4-NADH, at pD 7.2, 37 °C, and in the presence of air. The catalytic activity is markedly dependent on the arene, with the hexamethylbenzene (hmb) complex showing the highest activity. For [(η 6-hmb)Ru(en)Cl]PF6, the rate of reaction is independent of NAD+ concentration and shows saturation kinetics with respect to formate concentration. A K m value of 58 mM and a turnover frequency at saturation of 1.46 h−1 were observed. Removal of chloride and performing the reaction under argon led to higher reaction rates. Lung cancer cells (A549) were found to be remarkably tolerant to formate even at millimolar concentrations. The possibility of using ruthenium arene complexes coadministered with formate as catalytic drugs is discussed.Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users.
Keywords:Organometallic complex  Ruthenium  Arene  Catalysis  NAD
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号