Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium |
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| Authors: | S Sato H Murakami T Sugahara T Ikegami K Yamada H Omura S Hashizume |
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| Institution: | (1) Morinaga Institute of Biological Science, Shimosueyoshi 2-1-1, Tsurumi-ku, 230 Yokohama, Japan;(2) Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, 812 Fukuoka, Japan;(3) Shimadzu Co. Kuwabara-cho 1, Nishinokyo, Nakagyo-ku, 604 Kyoto, Japan |
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| Abstract: | Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1×105 cells/ml in dishes. At the higher cell density of 5×105 cells/ml, K-phosphate was more effective than Na-phosphate, at the same concentration. In large-scale continuous culture, the addition of 10 mM Na-phosphate into serum-free culture medium stimulated antibody production by HB4C5 cells 6-fold. |
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| Keywords: | human monoclonal antibody potassium phosphate sodium phosphate human-human hybridoma and serum-free medium |
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