抗体应答期间大鼠脑和胸腺中儿茶酚胺含量的变化

目的：探讨在抗体应答期间，脑和淋巴器官中儿茶酚胺（ＣＡｓ）含量的动态变化，籍以了解免疫状态对中枢和外周ＣＡｓ神经活动的影响。方法：用绵羊红细胞（ＳＲＢＣ）免疫大鼠，在免疫后第２￣７ｄ应用高效液相色谱－电化学检测法（ＨＰＬＣ－ＥＣＤ）测定大鼠下丘脑、海马、脑干和胸腺中云甲肾上腺素（ＮＡ）、肾上腺素（Ａ）、多巴胺（ＤＡ）和高香草酸（ＨＶＡ）的含量。结果：①下且脑和海马内ＮＡ在抗体应答期间升高，而胸腺中

CHANGES OF CATECHOLAMINE CONTENT IN THE BRAIN AND THYMUS OF RATS DURING ANTIBODY RESPONSE

AIM: The kinetic changes of catecholamine content in the brain and lymphoid organ of rats during the antibody response were investigated in order to know the effect of immune condition on the activity of catecholaminergic nerve in central and peripheral nervous system. METHODS: Rats were immunized with sheep red blood cells (SRBC). During the days 2-7 after immunization, the content of noradrenaline (NA), adrenaline (A), dopamine (DA) and homovanilic acid (HVA) in the hypothalamus, hippocampus, brainstem and thymus of rats was determined by means of the high-performance liquid chromatography with electrochemical detection. RESULTS: 1. NA content in the hypothalamus and hippocampus significantly increased, but was strikingly decreased in the thymus during the antibody response compared with the saline control. 2. The content in the hypothalamus, hippocampus and thymus markedly augmented in the period of antibody response. 3. DA content in the hypothalamus significantly increased, but in the hippocampus, DA content markedly descended after immunization. HVA, DA metabolite, dramatically rose in the hypothalamus and hippocampus. 4. There were not significant alterations of catecholamine content in the brainstem during the antibody response compared with the saline control. CONCLUSION: The antibody response may enhance the activity of catecholaminergic nerve in the hypothalamus and hippocampus, and may exert different impact on various catecholaminergic nerve in the thymus, but does not markedly affect the activity of catecholaminergic nerve in the brainstem.