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抗干扰微生物检测技术研究
引用本文:吴清平, 蔡芷荷, 张菊梅, 周小燕, 姚汝华,.抗干扰微生物检测技术研究[J].微生物学通报,2000,27(2):101-105.
作者姓名:吴清平  蔡芷荷  张菊梅  周小燕  姚汝华  
作者单位:1. 华南理工大学广州 510641;广东省微生物研究所广州 510070
2. 广东省微生物研究所广州 510070
3. 华南理工大学广州 510641
基金项目:广东省重点科技攻关项目
摘    要:在检测细菌、真菌和大肠菌群时,抗防腐剂型微生物培养基可消除样品中浓度为 2.0g/L(Kg)的山梨酸、山梨酸钾、苯甲酸及苯甲酸钠的干扰;抗消毒剂型微生物培养基可分别消除150mg/ L二氧化氯、400mp/ L过氧乙酸、700mg/ L次氯酸钠及180mp/ L过氧化氢的干扰;抗臭氧型微生物培养基可以消除10.0mg/L的臭氧和余氯的干扰。大样倾注平板法可以取5mL的样品,适用所有样品的检测;液体大样法可以取100mL的样品,适用于所有样品的检测及增菌,特别适用于无色液体样品;最小近似数法可以检出次大样中

关 键 词:抗干扰    检测技术    微生物培养基    大样倾注平板法    液体大样法  
文章编号:0253-2654(2000)02-0101-05
修稿时间:1999年1月11日

Researches on Anti-disturbance Microbial Measurement Techniques
WU Qing-Ping, CHAI Zhi-He, ZHANG Ju-Mei, ZHOU Xiao-Yan, RAO Ru-Hua.Researches on Anti-disturbance Microbial Measurement Techniques[J].Microbiology,2000,27(2):101-105.
Authors:WU Qing-Ping  CHAI Zhi-He  ZHANG Ju-Mei  ZHOU Xiao-Yan  RAO Ru-Hua
Abstract:In the bacterial, fungous and conform bacterial measurement,the anti-preservative media can eliminate the disturbances of the preservatives 2.0g/L(Kg) sorbic acid, potassium sorbate,benzoic acid and sodium benzoate in the samples, the anti-disinfectant media can eliminate respectively the disturbances of the disinfectants 150mg/L chloride dioxide, 400mg/L peroxyacetic acid. 700mg/L sodium hypochlorite and 180mp/L hydrogen peroxide, and the anti-ozone microbial media can eliminate the disturbances of 10.0mp / L ozone and free chlorine. The big sample pouring plate method can take 5mL sample, suitable for the microbial detection of all kinds of samples. The liquid big sample method can take 100mL sample, suitable for the microbial detection and increasing microbial number of all kinds of samples, especially suitable for the no color liquid samples. The The method can measure the total number of the bacteria, fungi and conform bacteria in the second big samples by applying fifteen tube method or nine tube method. It is suitable for the microbial measurement of all kinds of samples, especially suitable for the microbial measlesment of the samples with color.
Keywords:Anti-disturbance  Measurement technique  Microbial media  The big sample pouring plate method  The liquid big samples method
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