The conformation of the d(ACCCGGGT) duplex in aqueous solution |
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| Authors: | M A Keniry C Levenson R H Shafer |
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| Institution: | Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143. |
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| Abstract: | The nonexchangeable base and sugar protons of the octanucleotide d(ACCCGGGT)2 have been assigned using two dimensional homonuclear Hartmann-Hahn relayed spectroscopy (HOHAHA), double quantum filtered homonuclear correlation spectroscopy (DQFCOSY) and nuclear Overhauser spectroscopy (NOESY) in D2O at 12 degrees C. The observed NOE's between the base protons and their own H2' protons and between the base protons and the H2' protons of the 5' adjacent nucleotide and the observed coupling constants between the deoxyribose 1' and 2',2' protons indicate that this duplex assumes a right-handed B-type helix conformation in solution. |
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