白灵侧耳栽培种质对高温胁迫的反应研究

本文以“中农一号”、“华杂13”、“中农翅鲍”和“CCMSSC 00488”4个白灵侧耳菌株为材料,通过测定高温胁迫下菌丝体内硫代巴比妥酸反应物（thiobarbituric acid-reactive substances,TBARS）和蛋白质羰基（protein carbonyl,PCO）含量,观察适温培养和高温胁迫后恢复培养时菌落的生长速率、生长势和菌丝形态特征,研究不同白灵侧耳栽培种质对高温胁迫的反应。研究表明,白灵侧耳不同栽培种质对高温胁迫的反应在氧化损伤程度、菌落形态和菌丝形态特征上都有变化,不同材料的高温伤害程度有着较显著的差异。4个材料高温胁迫条件下TBARS和PCO含量均显著升高,但是升高程度不同,按照TBARS和PCO含量多少排序,依次为CCMSSC 00488>华杂13>中农翅鲍>中农一号。高温胁迫处理后恢复生长需要的时间依次为：CCMSSC 00488>华杂13>中农翅鲍、中农一号;菌落生长势：中农一号>中农翅鲍=华杂13>CCMSSC 00488;菌丝形态特征：菌丝表面增长率、菌丝顶端细胞表面积、菌丝直径和菌丝体分支频率均显著降低,但降低程度以中农一号为最大。菌丝体分支频率与菌落生长势相对应。

Heat stress response of cultivated Pleurotus eryngii var.tuoliensis germplasms

To investigate the heat stress response of four cultivated germplasms of Pleurotus eryngii var. tuoliensis (“Zhongnong No.1”, “Huaza 13”, “Zhongnongchibao” and “CCMSSC 00488”), the concentration of thiobarbituric acid-reactive substances (TBARS) and protein carbonyl (PCO) in mycelia was determined, and the colony growth rate, growth vigor and the hyphal morphological characters were detected. Results showed that the responses to heat stress differed in the level of oxidative damage, the colony character, and hyphal morphology, and significant differences of damage existed among the four different cultivated germplasms. The TBARS and PCO content were all significantly increased under heat stress but varied in degree for the four strains in order of CCMSSC 00488 > Huaza 13 > Zhongnongchibao > Zhongnong No.1. The growth recovery duration needed for the four strains after heat stress was ranked as CCMSSC 00488 > Huaza 13 > Zhongnongchibao, Zhongnong No.1, while the colony growth vigor was ranked as Zhongnong No.1 > Zhongnongchibao = Huaza 13 > CCMSSC 00488. The hyphal morphology character, surface growth rate, cell surface area of the distal hyphae, hyphal diameter, and mycelium branching frequency were all significantly reduced, and the strain Zhongnong No.1 suffered the most serious decline. The mycelium branching frequency was relevant to the colony growth vigor.