The effect of glucosinolates (mustard oil glycosides) and products of their enzymic degradation on the infectivity of turnip mosaic virus |
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Authors: | J ?pak L Kolá?ová J Lewis G R Fenwick |
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Institution: | 1. Institute of Plant Molecular Biology, Academy of Sciences of Czech Republic, Brani?ovská 31, 370 05, ?eské Budějovice, Czech Republic 2. AFRC Institute of Food Research, Norwich Laboratory, Colney Lane, NR 47 UA, Norwich, Great Britain
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Abstract: | The effect of glucosinolates sinigrin, progoitrin, epiprogroitrin, gluconapin, gluconapoleiferin, glucobrassicanapin, glucotropaeolin
and gluconasturtiin without and with the glucosinolate-degrading enzyme (myrosinase, EC:3.2.3.1.), on the infectivity of turnip
mosaic virus was studied. Little or no effect was observed when the intact glucosinolate (2.5 μmol cm−3) was added to the suspension of turnip mosaic virus (TuMV, isolate Ruzyně; 0.2 mg cm−3 in 0.01 M potassium phosphate buffer) at both pH 7 and pH 6. A significant decrease of virus infectivity was, however, observed
when 0.25, 1.25 and 2.5 μmol cm−3 of the glucosinolate together with 0.31, 1.56 and 3.13 mg cm−3 of the myrosinase, respectively, was added to the virus suspension of both pH 7 and pH 6. The effect, which was greater at
pH 6, was most intense with sinalbin and glucobrassicin substrates. |
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