RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS REVEAL CROSS-FERTILISATION IN BIOMPHALARIA GLABRATA (PULMONATA: BASOMMATOPHORA) |
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Authors: | VERNON JENNIFER G; JONES CATHERINE S; NOBLE LESLIE R |
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Institution: |
1Department of Zoology South Parks Road, Oxford OX1 3PS;
2Genetics Laboratory, Department of Biochemistry, South Parks Road, Oxford OXI 3QU; and
3Department of Zoology, Tillydrone Avenue, Aberdeen, AB9 2TN |
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Abstract: | The freshwater, schistosome-transmitting snail, Biomphalariaglabrata is a simultaneous hermaphrodite which can reproduceby both cross-and selffertilisation, but despite the medicalimportance of this species, little is known about the matingstrategies adopted by wild-type individuals from natural populations.The identification of cross-fertilised progeny is a prerequisitefor both evolutionary studies of the relative fitness of differentreproductive strategies, and for the production of exclusivelyoutcrossed F1 populations for genetic mapping of biologicallyimportant phenotypes, most notably, resistance to schistosomeinfection. In this study, parents, offspring and syntheticoffspring (controls) from four families of B. glabratarecently derived from wild populations were analysed using theRandom Amplified Polymorphic DNA (RAPD) assay. RAPDs were usedbecause they reveal more genetic variation, require less tissue(juvenile snails were only 45 mm diameter) than proteinelectrophoresis, are less laborious than other molecular analyses,and do not require sequence data. Seven of the 19 arbitrarysequence, oligonucleotide primers used gave bands that werepolymorophic between pairs of parent snails. Scoring the offspringfor the presence/absence of these polymorphic bands showed thatall the offspring tested were the products of cross-fertilisation.This study provides the first demonstration of the applicabilityof the RAPD technique to an analysis of fertilisation in wild-typeB. glabrata. (Received 20 December 1994; accepted 27 March 1995) |
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